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The aim of this course is to familiarize the participants with the primary analysis of RNA-seq data.

This course starts with a brief introduction to RNA-seq and discusses quality control issues. Next, we will present the alignment step, quantification of expression and differential expression analysis. For downstream analysis we will focus on tools available through the Bioconductor project for manipulating and analysing bulk RNA-seq.



  • Basic experience of command line UNIX
  • An introduction to the the Unix system and shell is available here.
  • Basic knowledge of the R syntax
  • For a beginner's introduction into R see here.


For additional information, follow this link.