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Bioinformatics Training

 

PhD candidate Gene Therapy and Proteomics at the University of Cambridge

Biography

Research in Kathryn Lilley’s group in collaboration with AstraZeneca. Currently using novel spatial proteomics approaches to investigate the mechanism by which adeno-associated viral vectors are secreted from HEK293-VP cells during manufacturing. The aim is to gain insight into the secretory pathway and to understand why some serotypes are secreted whilst others are retained within the cells. Such insight could lead to optimisation of manufacturing processes.

Education

  • University of Cambridge

    PhD Biochemistry

    2021 - 2025

    Using novel (expression and spatial) proteomics methods to investigate interactions of adeno-associated viral (AAV) vectors with HEK293-VP cells during manufacturing. Specifically, I am interested in determining how AAVs are secreted into the cell media, and why this secretion is only observed for certain AAV serotypes with others being retained within the cells.

    The primary motivation for my research is a collaboration with AstraZeneca to enhance the efficiency of AAV vector manufacturing. If AAVs were able to be harvested from cell media rather than lysates, fewer downstream processing and purification steps would lead to cheaper manufacturing with higher yields.

  • Imperial College London

    Master's degree Genes, Drugs and Stem Cells - Novel Therapies Distinction

    2020 - 2021

    MSc Academic Representative

    Research: 6-month project investigating polymeric carriers for delivery of an RNA therapeutic to pancreatic cancer cells. Specifically, evaluating cytotoxicity and transfection efficiency of different polymeric architectures.

  • Imperial College London

    Bachelor of Science - BSc Biological Sciences 1st Class Honours

    2017 - 2020

    Activities and Societies: Imperial College Data Science Society

    Literature Research 2nd Year: Review of electroceuticals as a novel approach to cancer management. Specifically, considering dopamine and acetylcholine pathways as potential targets for electrical stimulation.

    Literature Research 3rd Year: Review of the prospect of gene drives as a method of agricultural pest control. Specifically, the upcoming gene drive technologies (particularly CRISPR-based drives) were evaluated from a political, economic, social and technological perspective

Publications

Teaching and Supervisions

Teaching: 

 

2022/2023:

  • NST Part II BBS Bioinformatic, Computational Proteomics - Trainer

 

2023/2024:

  • Analysis of expression Proteomics data in R - Trainer
  • NST Part II BBS Bioinformatic, Computational Proteomics - Trainer
Charlotte Hutchings

Contact Details

Email address: 
The Lilley Group
Department of Biochemistry
c/o MRC Toxicology Unit
Gleeson Building
Tennis Court Road
Cambridge
CB2 1QR