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Research Informatics Training

 

These courses aim to familiarise the participants with advanced data analysis methodologies and provide hands-on training on the latest analytical approaches for specific types of biological data.

The analysis of high-throughput sequencing data is the most popular topic, as reflected by the wide range of courses covering this subject (e.g. RNA-seq, ChIP-seq, variation, DNA methylation, whole exome sequencing analysis) but the spectrum of training courses on offer is much wider.

Recommended starting point

 Introduction to the Unix Command Line

Practical introduction to the Linux command line and UNIX environment, including filesystem navigation, text processing and building simple command-line workflows.

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 Working on HPC Clusters

Introductory overview of high performance computing (HPC), including using shared computing clusters and job scheduling systems for large-scale computational analyses.

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Building on from introductory programming

 Bulk RNA-seq analysis

Practical introduction to bulk RNA sequencing data analysis, covering quality control, quantification of gene expression, exploratory analysis, differential expression, visualisation, and functional interpretation of results using R and Bioconductor.

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 Single-cell RNA-seq analysis.

Practical introduction to the analysis of single-cell RNA sequencing data, covering preprocessing, quality control, dimensionality reduction, data integration, clustering, and differential analysis using R/Bioconductor.

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 Working with Bacterial Genomes

Practical introduction to bacterial genomics analysis using Illumina sequencing data, covering genome assembly, annotation, phylogenetic analysis, strain typing, and antimicrobial resistance detection using standardised workflows and community tools.

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 Introduction to Mass Spectrometry: Theory and Applications

The aim of this course is to provide a comprehensive overview of mass spectrometry techniques, working principles and applications in STEM. Throughout the course, we will consider different ionization techniques and mass analyzers, hyphenation to chromatography or reaction coils, as well as upstream methodologies suitable for mass spectrometry in general. You will gain an understanding of what kind of data different mass spectrometry techniques provide and how to extract information from this data. This knowledge will enable you to plan and design mass spectrometry experiments for different applications.

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 Expression proteomics analysis in R

Practical introduction to the analysis of expression proteomics data in R and Bioconductor, covering data import, quality control, visualisation, differential abundance analysis, and biological interpretation of results.

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 Extracting biological information from gene lists

Practical introduction to gene set enrichment analysis, covering functional annotation resources, statistical methods for enrichment testing, web-based tools, and programmatic analysis of gene lists in R.

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 Quality Control in Sequencing Experiments

Practical introduction to quality control for short-read sequencing experiments, focusing on the interpretation of quality metrics and reports generated by commonly used quality assessment tools.

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 Metabolomics data analysis

Practical introduction to metabolomics, covering metabolite extraction, analytical technologies, metabolomics data analysis, metabolite identification, and interpretation of metabolic pathways.

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 Metagenomics data analysis

Practical introduction to metagenomics approaches for studying complex microbial communities, covering amplicon sequencing, shotgun and Hi-C metagenomics, quality control, assembly, and downstream analysis and interpretation of metagenomic data.

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 Foundations of Phylogenetic Inference

Practical introduction to molecular phylogenetics, covering sequence alignment, phylogenetic tree reconstruction, maximum likelihood and Bayesian inference methods, and interpretation of evolutionary relationships.

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 Building Computational Pipelines with Nextflow

Practical introduction to building reproducible and scalable computational pipelines using Nextflow and nf-core, covering workflow development, software management, modular pipeline design, and execution across local and high-performance computing environments.

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 Building Computational Pipelines with Snakemake

This 1-day workshop will cover the principles for building workflows using Snakemake, as well as more advanced strategies to fully customise, automate and scale your analysis.

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 Managing Bioinformatics Software and Pipelines

Practical introduction to managing bioinformatics software and automating analyses using package managers, software containers, and workflow management systems.

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 Protein Structure Prediction and Analysis

Practical introduction to computational protein structure prediction, covering structure prediction with AlphaFold, model evaluation, visualisation, multimer prediction, ligand binding site prediction, and molecular docking.

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 Prompting for biologists: using AI chatbots for effective data analysis

As the use of AI chatbots continues to rise, it is crucial to understand what they are, how they work, and how to make the most of them. Prompting is the method of interacting with AI, and as AI chatbots become more openly and widely available, a good and effective prompt could make all the difference.

In this course, we will provide background on the history of AI chatbots as well as an understanding of how they work. We will provide hands-on use cases of how to prompt like a bioinformatician/software engineer as well as providing strategies and tactics of prompting to unleash the full potential of AI chatbots in biological data analysis. This course is aimed at researchers with no computational background.

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 Spatial Transcriptomics Analysis

Practical introduction to the analysis of spatial transcriptomics data using Seurat and related R packages, covering preprocessing, normalisation, integration, clustering, visualisation, and inference of spatial organisation and cell-cell interactions.

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Other useful links

Data Carpentry