Puspendu Sardar, PhD

PhD-trained microbial geneticist with over 12 years of experience in computational biology, bio-statistics, and analysis of bioactive compounds. Advanced skill in R and BASH, meta-omics data analysis (meta-genomics and meta-transcriptomics) molecular evolution of proteins (Membrane protein, Enzymes, Scaffold protein). I have work experience in 4 different countries (India, Germany, the Czech Republic, and the UK) with colleagues from more than 15 nations.

Biography

Puspendu Sardar was an International Max Planck Research School (IMPRS) fellow and received his PhD in molecular genetics from the University of Kiel, Germany, in 2018 under the supervision of Prof. Dr Frank Kempken. He then joined the Czech Academy of Sciences as a postdoc and worked on microbial community composition in different environmental conditions. Since September 2021, Puspendu started his post-doctoral training with Dr Virginia Pedicord at the University of Cambridge, UK. Puspendu’s current research is focused on the understanding gut-microbial community in mouse tumour models and human cancer patients and its relation to host immunity.

Research

Specialized in:

Meta/genomics, meta/transcriptomics, comparative genomics, antimicrobial resistome profiling, and Next-Generation Sequencing (NGS) data analysis.
Identification of novel genes and enzymes, gene manipulation, metabolite profiling, fungal biology, and bio-synthetic network.
Molecular evolution and reconstruction of metabolic pathways, molecular techniques, structural analysis of biological macro-molecules, programming languages, and R.
Purification of natural fungal products, chromatography, RT/qRT-PCR, maintaining microbial cultures, microscopy, protein expression and purification, analytical instrumentation, and animal model.

Publications

Key publications:

Soil-specific responses in the antibiotic resistome of culturable Acinetobacter spp. and other non-fermentative Gram-negative bacteria following experimental manure application. Sardar P, Elhottová D, Pérez-Valera E. FEMS Microbiology Ecology. 2023; 99: fiad148		2023
Enterocloster clostridioformis induces host intestinal epithelial responses that protect against Salmonella infection BS Beresford-Jones, S Suyama, SA Clare, AT Soderholm, W Xia, ... bioRxiv, 2023.07. 20.549886		2023
De novo metatranscriptomic exploration of gene function in the millipede holobiont Sardar, P., Šustr, V., Chroňáková, A., Lorenc, F., Faktorová, L. Scientific Reports 12 (16173)		2022
Metatranscriptomic holobiont analysis of carbohydrate-active enzymes in the millipede Telodeinopus aoutii (Diplopoda, Spirostreptida) P Sardar, V Šustr, A Chroňáková, F Lorenc Frontiers in Ecology and Evolution 10, 931986		2022
Characterization of indole-3-pyruvic acid pathway-mediated biosynthesis of auxin in Neurospora crassa P Sardar, F Kempken PLoS One 13 (2), e0192293		2018
Influence of membrane cholesterol in the molecular evolution and functional regulation of TRPV4 S Kumari, A Kumar, P Sardar, M Yadav, RK Majhi, A Kumar, C Goswami Biochemical and Biophysical Research Communications 456 (1), 312-319		2015
BEM46 shows eisosomal localization and association with tryptophan-derived auxin pathway in Neurospora crassa K Kollath-Leiss, C Bönniger, P Sardar, F Kempken Eukaryotic Cell 13 (8), 1051-1063		2014
Molecular phylogeny of OVOL genes illustrates a conserved C2H2 zinc finger domain coupled by hypervariable unstructured regions A Kumar, A Bhandari, R Sinha, P Sardar, M Sushma, P Goyal, C Goswami, ... PloS one 7 (6), e39399		2012
Conservation of tubulin-binding sequences in TRPV1 throughout evolution P Sardar, A Kumar, A Bhandari, C Goswami PLoS One 7 (4), e31448		2012
Right time-right location-right move: TRPs find motors for common functions R Majhi, P Sardar, L Goswami, C Goswami Channels 5 (4), 375-381		2011